Lab News
July
CONGRATULATIONS to PROFESSOR XIAOYUAN HAN on her new appointment as an Assistant Professor at University of the Pacific, Arthur A. Dugoni School of Dentistry. We’re sad to see her go, but proud all the same.
June
[22-26 June 2019] 34th Congress of the International Society for Advancement of Cytometry, Vancouver, Canada| Come find us at Cyto 2019! Program book.
- Dr. Xiaoyuan Han: Differential Dynamics of the Maternal Immune System in Healthy Pregnancy and Preeclampsia | Sunday 23 June 2019, 11:30 | Ballroom A
- Dr. Laura Peterson: Deep Profiling of the Neonatal Immune System using CyTOF | Tuesday 25 June 2019, 10:50 | Meeting room 1
- Amy Tsai: A Year-Long Immune Profile of the Systemic Response in Acute Stroke Survivors | Wednesday 26 June 2019, 14:45 | Ballroom A
- Dr. Edward Ganio: Deep Immune Profile of Preoperative Glucocorticoid Administration in Patients Undergoing Surgery | Poster B155
- Amy won the 2019 Exceptional Student Award!
[21 June 2019]: Systemic Immunologic Consequences of Chronic Periodontitis | Journal of Dental Research | A high-dimensional analysis of intracellular signaling networks revealed immune system–wide dysfunctions differentiating patients with ChP from healthy controls. Importantly, the immune alterations associated with ChP were no longer detectable 3 wk after periodontal treatment. Our findings demarcate systemic and cell-specific immune dysfunctions in patients with ChP, which can be temporarily reversed by the local treatment of ChP.
[11 June 2019]: Differential Dynamics of the Maternal Immune System in Healthy Pregnancy and Preeclampsia | Frontiers in Immunology | Our paper’s out! Global readout of the dynamics of the maternal immune system early in pregnancy lay the groundwork for identifying clinically-relevant immune dysfunctions for the prediction and prevention of preeclampsia.
- Scope, Stanford Medicine: Predicting women at risk of preeclampsia before clinical symptoms
[9-11 June 2019] Annual Shock Meeting, Coronado, CA | Amy received the Dr. Mohammed Sayeed Presidential Travel Award to present her work at the Annual Shock Meeting. She presents on Sunday, 12:30 pm in Commodore ABCD in the Oral Presentations By and For New Investigators session.
May
[23 May 2019 ] OKU/Sutro Excellence Day, University of the Pacific, School of Dentistry | The keynote lecture, titled “Profiling the systemic immunological consequences of periodontitis with single cell resolution” was delivered by Dr. Dyani Gaudilliere, clinical assistant professor of Surgery (Plastic Surgery), and chief of Dental Medicine & Surgery, and Dr. Brice Gaudillaire, assistant professor of Anesthesiology, both from Stanford School of Medicine.
[16-20 May 2019] AUA/IARS/SOCCA, Montréal, Canada | Kosaka Award Finalist | Our work on the deep immune profile of preoperative glucocorticoid administration in patients undergoing surgery was a finalist for the Kosaka awards.
March
[12 March 2019]: A year-long immune profile of the systemic response in acute stroke survivors | Brain | Our paper on early differences in immunological responses to stroke that are associated with functional outcomes one year from stroke onset is published and available!
- Science News: Stroke victims with busy immune responses may also see mental declines
- Scope, Stanford Medicine| Needle in a haystack: Two days after stroke, a handful of blood cells reveal risk of dementia a year later
[12-16 March 2019] 66th Annual Scientific Meeting, Society for Reproductive Investigations, Paris, France | Program Book | Dr. Xiaoyuan Han presents Differential Dynamics of the Maternal Immune System in Healthy Pregnancy and Preeclampsia (Poster F-186).
[4 March 2019] MCHRI Seminar Series | Drs Brice Gaudilliere and Laura Peterson discuss the immune system dynamics in normal and pathological pregnancies and deep immune profiling of the neonatal immune system.
February
[Feb 2019] Dr. Ina Stelzer recieves Forschungsstipendium from the Deutsche Forschungsgemeinschaft.
January
[Jan 2019] Western Society of Pediatric Research, Carmel, California | Dr. Laura Peterson presents her work on neonatal outcomes.
Deep Immune Profiling of the Newborn Immune System
AAP Perinatal Symposium | Bonita Springs, Florida
[Nov 2018]
[Nov 2018]
Dr. Laura Peterson presents her work as a platform presentation.
Onset of Labor
Finalist | President’s Award for Excellence | Cyto2018, Prague, Czechia
[5/3/18]
[5/3/18]
Dr. Xiaoyuan Han presented the Onset of Labor at the 33rd Congress of the International Society for Advancement of Cytometry conference and was a finalist for the Presidential Award for Excellance
Assessment of Maternal Peripheral Immune System By Mass Cytometry to Predict the Onset of Labor
International Anesthesia Research Society | Chicago, Illinois, USA
[4/28/18]
[4/28/18]
Dr. Kazuo Ando presents his poster at the IARS conference in Chicago.
Children’s Health Research Institute
Stanford CHRI Symposium | [Nov 2018]
Dr. Laura Peterson presents her work at the inaugural CHRI symposium. Additionally, she received a grant to continue with her research.
Microgravity on Immune System
Cyto2018, Prague, Czechia | [5/2/18]
Dr. Brice Gaudilliere presents on the effects of Microgravity on the Human Immune system at the 33rd Congress of the International Society for Advancement of Cytometry. This project is in collaboration with the Hughes-Fulford group at UCSF.
Peripheral Immune Signature of Chronic Periodontitis
Annual Stanford Plastic Surgery Research Symposium | [4/17/18]
Amy Tsai presents updates on the Peripheral Immune Signature of Chronic Periodontal Disease.
Poster Session
Cyto2018, Prague, Czechia | [4/30/18]
Karim Djebali, Amy Tsai, and Benjamin Choisy (from left to right) presented at the 33rd Congress of the International Society for Advancement of Cytometry. Karim's poster is titled: Identifying a Peripheral Immune Signature of Chronic Periodontitis using Mass Cytometry. Amy's is: The Systemic Immune Profile of Stroke Recovery. Ben's is: Systematic Examination of Whole Blood Sample Handling Procedure for Analysis of Immune Cell Function.
Predicting Onset of Labor by Mass Cytometry
Society for Reproductive Investigation 65th Annual Scientific Meeting | San Diego, CA, USA
[3/6/18]
[3/6/18]
Dr. Xiaoyuan Han presents her project at the SRI conference in San Diego.
Deep Immune Profiling of an Arginine-Enriched Nutritional Intervention in Patients Undergoing Surgery
Journal of Immunology
[9/15/17]
[9/15/17]
Application of high-content immune profiling technologies has enormous potential to advance medicine. Whether these technologies reveal pertinent biology when implemented in interventional clinical trials is an important question. The beneficial effects of preoperative arginine-enriched dietary supplements (AES) are highly context specific, as they reduce infection rates in elective surgery, but possibly increase morbidity in critically ill patients. This study combined single-cell mass cytometry with the multiplex analysis of relevant plasma cytokines to comprehensively profile the immune-modifying effects of this much-debated intervention in patients undergoing surgery.
Multicenter Systems Analysis of Human Blood Reveals Immature Neutrophils in Males and During Pregnancy
Scope by Stanford Medicine
[9/5/17]
[9/5/17]
We found frequencies of immature forms of neutrophils in the blood of women during late pregnancy, thus showing novel sex differences in innate immunity and identify a common neutrophil signature in males and in pregnant women.
The Human Immune Clock of Pregnancy
Published in Science Immunology
[9/1/17]
[9/1/17]
Precisely timed immune adaptations reflect an immune clock of pregnancy in women delivering at term, which provides the analytical framework necessary for identifying immunological deviations implicated in pregnancy-related pathologies.
Check out what others have to say on this topic
Pregnancy Around the Clock
Anke Diemert | Trends in Molecular Medicine
[12/1/17]
[12/1/17]
"The insights will undoubtedly promote the testing of such a clock – possibly in synergy with other pacemakers – to potentially predict pregnancy complications." Anke Diemert, feto-maternal tolerance specialist, University of Hamburg
Immune “Clock” Could Help Detect Pregnancy Complications
American Association for the Advancement of Science
[8/29/17]
[8/29/17]
Juwon Song features findings from our paper in AAAS. "The new 'immune clock' of pregnancy could guide future researchers as they explore ways to prevent pregnancy-associated disorders, like preterm birth."
An Immunological Timeline for Pregnancy
The Scientist
[9/1/17]
[9/1/17]
"This methodology holds great potential for the authors to unravel the complex immunological changes that precede (and may predict) early miscarriage, preterm labour, [and] pre-eclampsia.” Lynne Sykes, clinical lecturer, Imperial College London
What to expect when you're expecting
Focus | Science Immunology
[9/1/17]
[9/1/17]
Dr. Pratip Chattopadhyay explains how we applied systems immunology to study human pregnancy.
An Immunological Timeline for Pregnancy
The Scientist
[9/1/17]
[9/1/17]
"This methodology holds great potential for the authors to unravel the complex immunological changes that precede (and may predict) early miscarriage, preterm labour, [and] pre-eclampsia.” Lynne Sykes, clinical lecturer, Imperial College London
Stanford scientists find the immune clock of pregnancy to help understand preterm birth
Scope by Stanford Medicine
[9/5/17]
[9/5/17]
Erin Digitale summarizes some of the findings from our paper on the timing human immune responses leading up to pregnancy.
Deep Immune Profiling in Trauma and Sepsis: Flow Is the Way to Go!
Critical Care Medicine
[9/1/17]
[9/1/17]
Brice Gaudilliere and Martin Angst highlight the importance and versatility of mass cytometry in understanding the biological immune response to trauma.
Best Basic Science Oral Presentation Award
Department of Plastic Surgery
[4/11/17]
[4/11/17]
Amy Tsai receives the award for Best Basic Science Oral Presentation for her talk on the Systemic Immune Signature of Periodontal Disease at the Annual Stanford Plastic Surgery Research Symposium.
Best Data Analysis Research Award
Department of Anesthesia, Perioperative and Pain Medicine
[5/22/17]
[5/22/17]
Quentin Baca received the Best Data Analysis Research Award at the Stanford Anesthesia Research Dinner for his work on predicting postoperative pain in a cohort of 1,008 patients undergoing spine surgery.
Dr. Xiaoyuan Han receives Stanford CHRI Postdoctoral Award
[2017-2018]
Project: Identifying a predictive immune signature of preterm birth with mass cytometry
Best Basic Science Award
Department of Anesthesia, Perioperative and Pain Medicine
[5/22/17]
[5/22/17]
The Gaudilliere group, along with Dr. Martin Angst and Dr. Nima Aghaeepour's group received the Best Basic Science Award at the Stanford Anesthesia Research Dinner for work on identifying an immune signature of pregnancy.
Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states
Nature Medicine
1/16/17]
1/16/17]
Despite clear differences in immune system responses and in the prevalence of autoimmune diseases between males and females, there is little understanding of the processes involved. In this study, we identified a gene signature of immature-like neutrophils, characterized by the overexpression of genes encoding for several granule-containing proteins, which was found at higher levels (up to 3-fold) in young (20–30 y old) but not older (60 to >89 y old) males compared with females.
The road ahead: Implementing mass cytometry in clinical studies, one cell at a time
Clinical Mass Cytometry
[1/5/17]
[1/5/17]
Quentin Baca highlights six major studies that apply mass cytometry in a clinically relevant manner, from isolating cancer cells to progressive statistical analysis tools for analyzing mass cytometry datasets.
Mapping the Fetomaternal Peripheral Immune System at Term Pregnancy
Journal of Immunology
[12/1/16]
[12/1/16]
Preterm labor and infections are the leading causes of neonatal deaths worldwide. During pregnancy, immunological cross talk between the mother and her fetus is critical for the maintenance of pregnancy and the delivery of an immunocompetent neonate. A precise understanding of healthy fetomaternal immunity is the important first step to identifying dysregulated immune mechanisms driving adverse maternal or neonatal outcomes. Examination of paired samples revealed differences in endogenous signaling tone that are unique to a mother and her offspring.
Brice Gaudilliere: The Development to Term
2016 ChildX Symposium
[April 2016]
[April 2016]
Brice Gaudilliere discusses the maternal immune system, a window into mechanisms of preterm birth, at the 2016 ChildX Symposium. ChildX is a dynamic, TED-style conference designed to inspire innovation that improves pediatric
and maternal health.
and maternal health.
Pre-operative immune signatures correlate with recovery from surgical trauma
Journal of Immunology
[5/1/15]
[5/1/15]
Traumatic injury produces a profound immune response that mobilizes both innate and adaptive branches of the immune system. In this study, we asked whether differences in patients’ pre-surgical immune states determined recovery. Activation of canonical signaling pathways was elicited in vitro, in pre-surgical patient samples. Single-cell evoked immune responses were analyzed in cell subsets spanning the entire immune system using mass cytometry. Surprisingly, the magnitude of these responses varied significantly across patients, thereby defining patient-specific “immune states”.
Implementing Mass Cytometry at the Bedside to Study the Immunological Basis of Human Diseases: Distinctive Immune Features in Patients with a History of Term or Preterm Birth
Cytometry
7/17/15]
7/17/15]
As interest in the “bedside” application of mass cytometry is growing, the delineation of relevant methodological issues is called for. This report uses a newly generated dataset to discuss important methodological considerations when mass cytometry is implemented in a clinical study. Specifically, the use of whole blood samples versus peripheral blood mononuclear cells (PBMCs) to analyze high-dimensional mass cytometry datasets are discussed. The results suggest that women predisposed to preterm birth may be prone to mount an exacerbated TLR4 response during the course of pregnancy.
Patient-specific Immune States before Surgery Are Strong Correlates of Surgical Recovery
Anesthesiology | Perioperative Medicine
[ December 2015]
[ December 2015]
Recently, strong immune correlates of surgical recovery were identified in patients shortly after surgery. However, the ability to interrogate a patient’s immune state before surgery and predict recovery is highly desirable in perioperative medicine.
Clinical recovery from surgery correlates with single-cell immune signatures
Published in Science Translational Medicine
[9/24/14] Gaudilliere et. al
[9/24/14] Gaudilliere et. al
The simultaneous analysis of 14,000 phosphorylation events in precisely phenotyped immune cell subsets revealed uniform signaling responses among patients, demarcating a surgical immune signature. The mechanistically derived immune correlates point to diagnostic signatures, and potential therapeutic targets, that could postoperatively improve patient recovery.
After Surgery, Predicting a Speedy Recovery
New York Times
[9/29/14]
[9/29/14]
“It’s a new frontier,” said Dr. Laurie Glimcher, dean of Weill Cornell Medical College, who was not involved in the research. If such a “signaling fingerprint” could predict recovery or relapse, “it’s an interesting and unusual example of what I would call precision medicine.”
Immune activity shortly after surgery holds big clue to recovery rate
Science Daily
[9/24/14]
[9/24/14]
Brice Gaudilliere, Nima Aghaeepour, and Martin Angst collaboratively found that they could predict how well a patient would recover from surgery, based on the activity of a specific set of immune cells during the first 24 hours after surgery.
Immune Signatures Predict Surgery Recovery
Spotlight | Fluidigm
Stanford’s Brice Gaudillière, MD, PhD, is doing groundbreaking research in collaboration with Gabriela Fragiadakis, Robert Bruggner, Martin Angst and Garry Nolan to reveal how activation of specific cell transcription factors correlates with the speed of recovery after surgery.
